In situ cultivation of deep-sea water with bicarbonate fueled a different microbial community

Yong Wang Jun Li Zhanfei Wei Qingmei Li Yingli Zhou Wenli Li Jun Chen Suixue Wang Yongzhi Xin Aiqun Zhang

Yong Wang, Jun Li, Zhanfei Wei, Qingmei Li, Yingli Zhou, Wenli Li, Jun Chen, Suixue Wang, Yongzhi Xin, Aiqun Zhang. In situ cultivation of deep-sea water with bicarbonate fueled a different microbial community[J]. Acta Oceanologica Sinica, 2022, 41(12): 98-104. doi: 10.1007/s13131-021-1959-z
Citation: Yong Wang, Jun Li, Zhanfei Wei, Qingmei Li, Yingli Zhou, Wenli Li, Jun Chen, Suixue Wang, Yongzhi Xin, Aiqun Zhang. In situ cultivation of deep-sea water with bicarbonate fueled a different microbial community[J]. Acta Oceanologica Sinica, 2022, 41(12): 98-104. doi: 10.1007/s13131-021-1959-z

doi: 10.1007/s13131-021-1959-z

In situ cultivation of deep-sea water with bicarbonate fueled a different microbial community

Funds: The Hainan Provincial Natural Science Foundation of China under contract No. 322CXTD531; the National Key Research and Development Program of China under contract Nos 2018YFC0310005 and 2016YFC0302504.
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  • Figure  1.  Diagram illustrating deep-sea in situ cultivation of microbes with bicarbonate. The cultivation was started with pumping about 40 L deep-sea water into a plastic bag, followed by injection of 40 mL 100 mmol/L NaHCO3. After 40 min, the water was filtered thought a working unit of multiple in situ nucleic acids collection (MISNAC). The microbes on the membrane in the filtration chamber were broken by lysis buffer. The nucleic acids in cell lysate were precipitated by ethanol and collected by a polymer column.

    Figure  2.  Microbial structures at genus level. Taxonomic classification of amplicon sequence variants at 97% similarity was performed by comparing with references sequences of SILVA database (www.arb-silva.de/). The samples were referred to Table 1. The percentage and taxonomic names of the genera were shown in Table S2.

    Figure  3.  Principal Coordinates Analysis (PCoA) analysis of microbial communities. The PCoA analysis was performed using the microbial communities at genus level. The samples were referred to Table 1.

    Figure  4.  Phylogenetic inference of Asprobacter sp. The phylogenetic tree was constructed with aligned 16S rRNA fragments using maximum-likelihood algorithm. The bootstrap values larger than 90 were shown on the branches. Species name or isolate source was next to accession number of the sequence in NCBI.

    Table  1.   Information of sampling methods and sites

    SampleMethodDeploymentDepth/mLatitudeLongitudeTime
    FH18-NNiskinFH181 06917°31.203'N110°25.880'E2019. 5. 21 12:30
    FH22-NNiskinFH221 04817°31.337'N110°27.522'E2019. 5. 24 17:30
    FH21-5MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 22 22:00–23:00
    FH21-6MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 22 23:30–1:30
    FH21-7MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 23 2:00–3:30
    FH21-8MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 23 4:00–5:00
    FH21-9MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 23 5:30–6:30
    FH21-10MISNACFH211 03017°30.729'N110°25.880'E2019. 5. 23 7:00–8:00
    FH21-CCultivation
    +MISNAC
    FH211 03017°30.729'N110°25.880'E2019. 5. 23 8:00–10:00
    Note: MISNAC means multiple in situ nucleic acids collection.
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出版历程
  • 收稿日期:  2021-07-30
  • 录用日期:  2021-11-05
  • 网络出版日期:  2022-09-02
  • 刊出日期:  2022-12-30

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