HAN Yinglun, LI Jun, XU Lei, MA Qinghua, GOU Meng, PANG Yue, LIU Xin, LI Qingwei. Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica[J]. Acta Oceanologica Sinica, 2017, 36(12): 24-30. doi: 10.1007/s13131-017-1095-y
Citation: HAN Yinglun, LI Jun, XU Lei, MA Qinghua, GOU Meng, PANG Yue, LIU Xin, LI Qingwei. Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica[J]. Acta Oceanologica Sinica, 2017, 36(12): 24-30. doi: 10.1007/s13131-017-1095-y

Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica

doi: 10.1007/s13131-017-1095-y
  • Received Date: 2016-03-13
  • The Guanine nucleotide exchange factor Vav2 (Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases. In the current study, an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica). To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav. It was found that Vav2 genes were only existed in vertebrates and Lj-Vav2 was the original one found in agnathans. The evolutionary dynamics of conserved motifs of Vav2 were explored using combined amino acid sequence as markers, and it is revealed that the Calponin homology (CH) domain, Dbl-homologous (DH) domain, Pleckstrin homology (PH) domain, Cysteine-rich (C1) domains, Src homology 3 (SH3) domains and Src homology 2 (SH2) domain were conserved throughout the Vav2 gene family in vertebrates during gene evolution. Relative quantitative real-time PCR analysis showed that the Lj-Vav2 was distributed in the heart, kidney, supraneural myeloid body, liver, gill and lymphocyte-like cells. The Lj-Vav2 was found to be expressed in these tissues, and the level of which was upregulated in lymphocyte-like cells after the animal was stimulated with LPS. These results indicated that the Lj-Vav2 might be involved in the immune response of lymphocyte-like cells in lamprey. Meanwhile, our findings provided a foundation for further investigation of the function of Lj-Vav2 in the primary vertebrate.
  • loading
  • Bailey T L, Elkan C. 1994. Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proc Int Conf Intell Syst Mol Biol, 2: 28-36
    Cooper M D, Alder M N. 2006. The evolution of adaptive immune systems. Cell, 124(4): 815-822
    Foucault I, Le Bras S, Charvet C, et al. 2005. The adaptor protein 3BP2 associates with VAV guanine nucleotide exchange factors to regulate NFAT activation by the B-cell antigen receptor. Blood, 105(3): 1106-1113
    Guo Peng, Hirano M, Herrin B R, et al. 2008. Dual nature of the adaptive immune system in lampreys. Nature, 459(7248): 796-801
    Huang Huiqing, Huang Shengfeng, Yu Yingcai, et al. 2011. Functional characterization of a ficolin-mediated complement pathway in amphioxus. J Biol Chem, 286(42): 36739-36748
    Kasamatsu J, Sutoh Y, Fugo K, et al. 2010. Identification of a third variable lymphocyte receptor in the lamprey. Proc Natl Acad Sci USA, 107(32): 14304-14308
    Kumar S, Nei M, Dudley J, et al. 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform, 9(4): 299-306
    López-Lago M, Lee H, Cruz C, et al. 2000. Tyrosine phosphorylation mediates both activation and downmodulation of the biological activity of Vav. Mol Cell Biol, 20(5): 1678-1691
    Romero F, Fischer S. 1996. Structure and function of Vav. Cell Signal, 8(8): 545-553
    Tsutsui S, Nakamura O, Watanabe T. 2007. Lamprey (Lethenteron japonicum) IL-17 upregulated by LPS-stimulation in the skin cells. Immunogenetics, 59(11): 873-882
    Wu Fenfang, Zhao Jing, Chen Liyong, et al. 2012. A novel BTK-like protein involved in immune response in Lethenteron japonicum. Immunol Lett, 146(1-2): 57-63
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (812) PDF downloads(806) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return