Volume 39 Issue 10
Oct.  2020
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Yuan He, Yuantu Ye, Songdong Shen. Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera[J]. Acta Oceanologica Sinica, 2020, 39(10): 50-57. doi: 10.1007/s13131-020-1577-1
Citation: Yuan He, Yuantu Ye, Songdong Shen. Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera[J]. Acta Oceanologica Sinica, 2020, 39(10): 50-57. doi: 10.1007/s13131-020-1577-1

Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera

doi: 10.1007/s13131-020-1577-1
Funds:  The National Key R&D Program of China under contract No. 2016YFC1402102; the MNR Key Laboratory of Eco-Environmental Science and Technology, China under contract No. MEEST-2020-2; the Jiangsu Planned Projects for Postdoctoral Research Funds; the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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  • Corresponding author: E-mail: shensongdong@suda.edu.cn
  • Received Date: 2019-11-22
  • Accepted Date: 2020-01-03
  • Available Online: 2020-12-28
  • Publish Date: 2020-10-25
  • Ulva prolifera is a green alga that plays an important role in green tides. Carotenoid biosynthesis is a basic terpenoid metabolism that is very important for maintaining normal life activities in algae. In this study, we first reported the complete sequences of all genes in the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, which is the only carotenoid synthesis pathway in U. prolifera. Then, we compared these genes with those of other species. Additionally, by detecting the carotenoid contents and expression levels of key genes participating in carotenoid biosynthesis in U. prolifera under three different light (1 000 lx, 5 000 lx and 12 000 lx) and salinity (12, 24 and 40) regimes, we found that carotenoid synthesis could be influenced by light and salinity, such that low light and high salinity could promote the synthesis of carotenoids. The results showed that the expression levels of genes involved in the MEP and the downstream pathway could affect the biosynthesis of carotenoids at the molecular level. This study contributes to a better understanding of the roles of genes participating in carotenoid biosynthesis in U. prolifera and the environmental regulation of these genes.
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  • [1]
    Astley S B, Hughes D A, Wright A J A, et al. 2004. DNA damage and susceptibility to oxidative damage in lymphocytes: effects of carotenoids in vitro and in vivo. British Journal of Nutrition, 91(1): 53–61. doi: 10.1079/BJN20031028
    Bennett R N, Wallsgrove R M. 1994. Secondary metabolites in plant defence mechanisms. New Phytologist, 127(4): 617–633. doi: 10.1111/j.1469-8137.1994.tb02968.x
    Collins A R. 2001. Carotenoids and genomic stability. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 475(1–2): 21–28. doi: 10.1016/S0027-5107(01)00071-9
    Davies F K, Jinkerson R E, Posewitz M C. 2015. Toward a photosynthetic microbial platform for terpenoid engineering. Photosynthesis Research, 123(3): 265–284. doi: 10.1007/s11120-014-9979-6
    de Carvalho Gonçalves J F, Marenco R A, Vieira G. 2001. Concentration of photosynthetic pigments and chlorophyll fluorescence of mahogany and Tonka bean under two light environments. Revista Brasileira de Fisiologia Vegetal, 13(2): 149–157. doi: 10.1590/S0103-31312001000200004
    Duan Chuanren, Wang Bochu, Xu Shirong. 2003. The effects of the environment stress on the plant secondary metabolites. Journal of Chongqing University (in Chinese), 26(10): 67–71
    Gao Shan, Chen Xiaoyuan, Yi Qianqian, et al. 2010. A strategy for the proliferation of Ulva prolifera, main causative species of green tides, with formation of sporangia by fragmentation. PLoS One, 5(1): e8571. doi: 10.1371/journal.pone.0008571
    Guedes A C, Amaro H M, Malcata F X. 2011. Microalgae as sources of carotenoids. Marine Drugs, 9(4): 625–644. doi: 10.3390/md9040625
    He Yuan, Ma Yafeng, Du Yu, et al. 2018. Differential gene expression for carotenoid biosynthesis in a green alga Ulva prolifera based on transcriptome analysis. BMC Genomics, 19: 916. doi: 10.1186/s12864-018-5337-y
    He Yuan, Yan Zhihong, Du Yu, et al. 2017. Molecular cloning and expression analysis of two key genes, HDS and HDR, in the MEP pathway in Pyropia haitanensis. Scientific Reports, 7: 17499. doi: 10.1038/s41598-017-17521-9
    Kliebenstein D J. 2004. Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant, Cell & Environment, 27(6): 675–684
    Lange B M, Rujan T, Martin W, et al. 2000. Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes. Proceedings of the National Academy of Sciences of the United States of America, 97(24): 13172–13177. doi: 10.1073/pnas.240454797
    Lin Apeng, Shen Songdong, Wang jianwei, et al. 2008. Reproduction diversity of Enteromorpha prolifera. Journal of Integrative Plant Biology, 50(5): 622–629. doi: 10.1111/j.1744-7909.2008.00647.x
    Lohr M, Schwender J, Polle J E W. 2012. Isoprenoid biosynthesis in eukaryotic phototrophs: a spotlight on algae. Plant Science, 185–186: 9–22. doi: 10.1016/j.plantsci.2011.07.018
    Mikami K, Hosokawa M. 2013. Biosynthetic pathway and health benefits of fucoxanthin, an algae-specific xanthophyll in brown seaweeds. International Journal of Molecular Sciences, 14(7): 13763–13781. doi: 10.3390/ijms140713763
    Patias L D, Fernandes A S, Petry F C, et al. 2017. Carotenoid profile of three microalgae/cyanobacteria species with peroxyl radical scavenger capacity. Food Research International, 100: 260–266. doi: 10.1016/j.foodres.2017.06.069
    Rivasseau C, Seemann M, Boisson A M, et al. 2009. Accumulation of 2-C-methyl-D-erythritol 2, 4-cyclodiphosphate in illuminated plant leaves at supraoptimal temperatures reveals a bottleneck of the prokaryotic methylerythritol 4-phosphate pathway of isoprenoid biosynthesis. Plant, Cell & Environment, 32(1): 82–92
    Sharma E, Anand G, Kapoor R. 2017. Terpenoids in plant and arbuscular mycorrhiza-reinforced defence against herbivorous insects. Annals of Botany, 119(5): 791–801
    Shi Peng, Cao Hongxing, Li Dongxia, et al. 2016. Bioinformatics analysis of DXS gene from six tropical plants including oil palm (Elaeis guineensis Jacq). Guihaia (in Chinese), 36(4): 471–478
    Soto G, Stritzler M, Lisi C, et al. 2011. Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation. Journal of Experimental Botany, 62(15): 5699–5711. doi: 10.1093/jxb/err287
    Sun Guohua, Sui Zhenghong, Zhang Xuecheng. 2008. Cloning and characterization of the phytoene desaturase (pds) gene—a key enzyme for carotenoids synthesis in Dunaliella (Chlorophyta). Journal of Ocean University of China, 7(3): 311–318. doi: 10.1007/s11802-008-0311-y
    Takaichi S. 2011. Carotenoids in algae: distributions, biosyntheses and functions. Marine Drugs, 9(6): 1101–1118. doi: 10.3390/md9061101
    Vranová E. 2012. Systems understanding of isoprenoid pathway regulation in Arabidopsis. In: Bach T J, Rohmer M, eds. Isoprenoid Synthesis in Plants and Microorganisms. New York: Springer, 475-491
    Vranová E, Coman D, Gruissem W. 2013. Network analysis of the MVA and MEP pathways for isoprenoid synthesis. Annual Review of Plant Biology, 64: 665–700. doi: 10.1146/annurev-arplant-050312-120116
    Wang Qian, Pi Yan, Hou Rong, et al. 2008. Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(e)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. BMB Reports, 41(2): 112–118. doi: 10.5483/BMBRep.2008.41.2.112
    Xiao Jie, Zhang Xiaohong, Gao Chunlei, et al. 2016. Effect of temperature, salinity and irradiance on growth and photosynthesis of Ulva prolifera. Acta Oceanologica Sinica, 35(10): 114–121. doi: 10.1007/s13131-016-0891-0
    Yang Jianming, Guo Lizhong. 2014. Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways. Microbial Cell Factories, 13: 160. doi: 10.1186/s12934-014-0160-x
    Yang Lien, Huang Xingqi, Lu Qinqin, et al. 2016. Cloning and characterization of the geranylgeranyl diphosphate synthase (GGPS) responsible for carotenoid biosynthesis in Pyropia umbilicalis. Journal of Applied Phycology, 28(1): 671–678. doi: 10.1007/s10811-015-0593-6
    Yuan Jianping, Peng Juan, Yin Kai, et al. 2011. Potential health-promoting effects of astaxanthin: a high-value carotenoid mostly from microalgae. Molecular Nutrition & Food Research, 55(1): 150–165
    Zhang Cui, Lu Jian, Wu Jun, et al. 2017. Removal of phenanthrene from coastal waters by green tide algae Ulva prolifera. Science of the Total Environment, 609: 1322–1328. doi: 10.1016/j.scitotenv.2017.07.187
    Zhang Huawei, Ma Jiahai, Hu Xiang, et al. 2011. Reproductive characteristics of the floating algae in green tide. Journal of Shanghai Ocean University (in Chinese), 20(4): 600–606
    Zhang Baoyu, Zhu Daling, Wang Guangce, et al. 2014. Characterization of the AOX gene and cyanide-resistant respiration in Pyropia haitanensis (rhodophyta). Journal of Applied Phycology, 26(6): 2425–2433. doi: 10.1007/s10811-014-0274-x
    Zhao Jin, Jiang Peng, Qiu Ri, et al. 2018. The yellow sea green tide: a risk of macroalgae invasion. Harmful Algae, 77: 11–17. doi: 10.1016/j.hal.2018.05.007
    Zheng Jiawen, Li Zhuosi, Manabe Y, et al. 2018. Siphonaxanthin, a carotenoid from green algae, inhibits lipogenesis in hepatocytes via the suppression of liver x receptor α activity. Lipids, 53(1): 41–52. doi: 10.1002/lipd.12002
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