Volume 39 Issue 10
Oct.  2020
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Jing Wu, Yuexia Zhou, Xiaofei Liu, Yuan Cao, Chengjin Hu, Yingjian Chen. Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS[J]. Acta Oceanologica Sinica, 2020, 39(10): 140-146. doi: 10.1007/s13131-020-1635-8
Citation: Jing Wu, Yuexia Zhou, Xiaofei Liu, Yuan Cao, Chengjin Hu, Yingjian Chen. Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS[J]. Acta Oceanologica Sinica, 2020, 39(10): 140-146. doi: 10.1007/s13131-020-1635-8

Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS

doi: 10.1007/s13131-020-1635-8
Funds:  The Major PLA Research Project of “the 12th Five-year Plan”for Medical Science Develpment under contract No. BWS12J014.
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  • Corresponding author: E-mail: yjqchen@126.com
  • Received Date: 2019-10-21
  • Accepted Date: 2019-12-13
  • Available Online: 2020-12-28
  • Publish Date: 2020-10-25
  • Rapid and accurate identification of Vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires two or more days to complete. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has become a powerful tool for rapidly distinguishing between related bacterial species. However, its accuracy depends on the number of strains in a database. In the current study, we extend and apply the Vibrio database based on MALDI-TOF MS. A total of 74 strains of Vibrio representing 28 species were identified and included in new database. A phylogenetic tree based on rpoB sequence and dendrograms were constructed. We analyzed 30 clinical Vibrio of three species to evaluate the new database and carried out PCA dendrogram analyses for differences of strains. We created a new database that offered fast and accurate Vibrio identification. MSP and PCA dendrogram analyses provided technical support to track sources and incidences of Vibrio infection. In addition, the discovery of characteristic and differential peaks is useful for the future identification of Vibrio. This represents a powerful tool for the rapid and accurate classification and identification of Vibrio and closely related species.
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  • [1]
    Calderaro A, Gorrini C, Piccolo G, et al. 2014. Identification of Borrelia species after creation of an in-house MALDI-TOF MS database. PLoS One, 9(2): e88895. doi: 10.1371/journal.pone.0088895
    Calderaro A, Piccolo G, Montecchini S, et al. 2013. MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension. Journal of Proteomics, 78: 273–280. doi: 10.1016/j.jprot.2012.09.027
    Cheng W C, Jan I S, Chen J M, et al. 2015. Evaluation of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of Vibrio species. Journal of Clinical Microbiology, 53(5): 1741–1744. doi: 10.1128/JCM.00105-15
    Emami K, Askari V, Ullrich M, et al. 2012. Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. PLoS One, 7(6): e38515. doi: 10.1371/journal.pone.0038515
    Fagerquist C K, Garbus B R, Miller W G, et al. 2010. Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomics. Analytical Chemistry, 82(7): 2717–2725. doi: 10.1021/ac902455d
    Hazen T H, Martinez R J, Chen Y F, et al. 2009. Rapid identification of Vibrio parahaemolyticus by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Applied and Environmental Microbiology, 75(21): 6745–6756. doi: 10.1128/AEM.01171-09
    Huang C H, Huang L, Chang M T, et al. 2016. Establishment and application of an analytical in-house database (IHDB) for rapid discrimination of Bacillus subtilis group (BSG) using whole-cell MALDI-TOF MS technology. Molecular and Cellular Probes, 30(5): 312–319. doi: 10.1016/j.mcp.2016.08.002
    Kaneko T, Colwell R R. 1974. Distribution of Vibrio parahaemolyticus and related organisms in the Atlantic Ocean off South Carolina and Georgia. Applied and Environmental Microbiology, 28(6): 1009–1017. doi: 10.1128/AEM.28.6.1009-1017.1974
    Kaleta E J, Clark A E, Cherkaoui A, et al. 2011. Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. Clinical Chemistry, 57(7): 1057–1067. doi: 10.1373/clinchem.2011.161968
    Ki J S, Zhang R, Zhang W, et al. 2009. Analysis of RNA polymerase beta subunit (rpoB) gene sequences for the discriminative power of marine Vibrio species. Microbial Ecology, 58(4): 679–691. doi: 10.1007/s00248-009-9519-7
    Price N P J, Rooney A P, Swezey J L, et al. 2007. Mass spectrometric analysis of lipopeptides from Bacillus strains isolated from diverse geographical locations. FEMS Microbiology Letters, 271(1): 83–89. doi: 10.1111/j.1574-6968.2007.00702.x
    Shao Zongze. 2010. Catalogue of China Marine Microbial Collections (in Chinese). Beijing: Chemical Industry Press
    Seng P, Drancourt M, Gouriet F, et al. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clinical Infectious Diseases, 49(4): 543–551. doi: 10.1086/600885
    Singhal N, Kumar M, Kanaujia P K, et al. 2015. MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Frontiers in Microbiology, 6: 791
    Sogawa K, Watanabe M, Sato K, et al. 2012. Rapid identification of microorganisms by mass spectrometry: improved performance by incorporation of in-house spectral data into a commercial database. Analytical and Bioanalytical Chemistry, 403(7): 1811–1822. doi: 10.1007/s00216-011-5656-1
    Tarr C L, Patel J S, Puhr N D, et al. 2007. Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination. Journal of Clinical Microbiology, 45(1): 134–140. doi: 10.1128/JCM.01544-06
    Tiruvayipati S, Bhassu S. 2016. Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium. Gut Pathogens, 8(1): 15. doi: 10.1186/s13099-016-0097-1
    Xuan Guanhua, Jia Juntao, Chen Ying, et al. 2015. Strain-level visualized analysis of cold-stressed Vibrio parahaemolyticus based on MALDI-TOF mass fingerprinting. Microbial Pathogenesis, 88: 16–21. doi: 10.1016/j.micpath.2015.08.002
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