Volume 41 Issue 3
Mar.  2022
Turn off MathJax
Article Contents
Konglin Zhou, Yuping Gu, Lu Wang, Jianming Chen. Discovery of Cladonema multiramosum sp. nov. (Cnidaria: Hydrozoa: Cladonematidae) using DNA barcoding and life cycle analyses[J]. Acta Oceanologica Sinica, 2022, 41(3): 44-52. doi: 10.1007/s13131-021-1900-5
Citation: Konglin Zhou, Yuping Gu, Lu Wang, Jianming Chen. Discovery of Cladonema multiramosum sp. nov. (Cnidaria: Hydrozoa: Cladonematidae) using DNA barcoding and life cycle analyses[J]. Acta Oceanologica Sinica, 2022, 41(3): 44-52. doi: 10.1007/s13131-021-1900-5

Discovery of Cladonema multiramosum sp. nov. (Cnidaria: Hydrozoa: Cladonematidae) using DNA barcoding and life cycle analyses

doi: 10.1007/s13131-021-1900-5
Funds:  The Fund of China Ocean Mineral Resources R&D Association under contract No. DY135-B2-13; the National Natural Science Foundation of China under contract No. 41806181; the Natural Science Foundation of Fujian Province, China under contract No. 2021J011042; the Program for Innovative Research Team in Science & Technology in Fujian Province University; the Talent Introduction Pre-research Project of Minjiang University under contract No. MJY20017.
More Information
  • Corresponding author: zhoukl@ mju.edu.cn; chenjm@mju.edu.cn
  • Received Date: 2021-07-25
  • Accepted Date: 2021-09-13
  • Available Online: 2021-12-01
  • Publish Date: 2022-03-01
  • In contrast to typical planktonic hydromedusae, Cladonema medusae are mostly benthic, with specialised adhesive branches to adhere to the substrate. In this study, a Cladonema species discovered in a laboratory aquarium in Fuzhou, China, was confirmed as a new species, based on morphological and molecular analyses. The species was named Cladonema multiramosum sp. nov. Its medusa is distinct from that of congeners possessing substantially more adhesive branches (8–24, rarely 5–7), and tiny branches on the upper radial canals. The validity of C. multiramosumum sp. nov. was also supported by molecular phylogenetic analyses based on the mitochondrial 16S rDNA sequence. However, C. multiramosum sp. nov. medusa also displayed considerable phenotypic plasticity with respect to its radial canals, tentacles, stinging branches per tentacle, oral tentacles, manubrium, and gonads, hindering species identification based solely on morphology. Although some morphological characteristics of hydroids (filiform tentacles and medusa buds) and nematocysts could also be used as diagnostic characters in the genus Cladonema, this information is unavailable for some Cladonema species. Thus, the taxonomy within the genus Cladonema was re-evaluated based mainly on the morphological characteristics of the medusa. Further revision of the genus Cladonema should be made when supplementary information on the life cycle and DNA barcoding are updated.
  • loading
  • [1]
    Bouillon J, Boero F, Seghers G. 1988. Notes additionelles sur les Hydroméduses de la mer de Bismarck (Hydrozoa-Cnidaria) II. Indo-Malayan Zoology, 5: 87–99
    [2]
    Bouillon J, Gravili C, Pagès F, et al. 2006. An introduction to Hydrozoa. Memoires du Museum National d'Histoire Naturelle, 194: 591
    [3]
    Bucklin A, Hopcroft R R, Kosobokova K N, et al. 2010a. DNA barcoding of Arctic Ocean holozooplankton for species identification and recognition. Deep-Sea Research Part II: Topical Studies in Oceanography, 57(1–2): 40–48
    [4]
    Bucklin A, Ortman B D, Jennings R M, et al. 2010b. A “Rosetta Stone” for metazoan zooplankton: DNA barcode analysis of species diversity of the Sargasso Sea (Northwest Atlantic Ocean). Deep-Sea Research Part II: Topical Studies in Oceanography, 57(24–26): 2234–2247
    [5]
    Cedeño-Posso C. 2014. First record of the genus Cladonema (medusae and polyps) in Colombia. Zootaxa, 3793(5): 597–599. doi: 10.11646/zootaxa.3793.5.8
    [6]
    Ender A, Schierwater B. 2003. Placozoa are not derived cnidarians: evidence from molecular morphology. Molecular Biology and Evolution, 20(1): 130–134
    [7]
    Farias G B, Leitão S N, De Castro Melo P A M, et al. 2020. First in situ record of the medusa stage of Cladonema radiatum (Cnidaria: Anthoathecata) in the South Atlantic Ocean. Ocean and Coastal Research, 68: e20349. doi: 10.1590/s2675-28242020068349
    [8]
    Folmer O, Black M B, Hoeh W R, et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5): 294–299
    [9]
    Fujiki A, Hou Shiting, Nakamoto A, et al. 2019. Branching pattern and morphogenesis of medusa tentacles in the jellyfish Cladonema pacificum (Hydrozoa, Cnidaria). Zoological Letters, 5(1): 12. doi: 10.1186/s40851-019-0124-4
    [10]
    Fujita S, Kuranaga E, Nakajima Y I. 2019. Cell proliferation controls body size growth, tentacle morphogenesis, and regeneration in hydrozoan jellyfish Cladonema pacificum. PeerJ, 7: e7579. doi: 10.7717/peerj.7579
    [11]
    Gershwin L A, Zeidler W. 2008. Cladonema timmsii, a new species of hydromedusa (Cnidaria: Hydrozoa) from a salt lake in South Australia. Zootaxa, 1826(1): 59–68. doi: 10.11646/zootaxa.1826.1.4
    [12]
    Ghory F S, Ali Q M, Ahmed Q. 2020. First record of Cladonema radiatum Dujardin, 1843 (Hydrozoa: Cladonematidae) from northern Arabian Sea, Pakistan. International Journal of Fisheries and Aquatic Research, 5(3): 14–16
    [13]
    Graziussi D F, Suga H, Schmid V, et al. 2012. The “Eyes absent” (eya) gene in the eye-bearing Hydrozoan Jellyfish Cladonema radiatum: conservation of the retinal determination network. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 318(4): 257–267. doi: 10.1002/jez.b.22442
    [14]
    Guindon S, Dufayard J F, Lefort V, et al. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59(3): 307–321. doi: 10.1093/sysbio/syq010
    [15]
    He Jinru, Zheng Lianming, Zhang Wenjing, et al. 2015. Morphology and molecular analyses of a new Clytia species (Cnidaria: Hydrozoa: Campanulariidae) from the East China Sea. Journal of the Marine Biological Association of the United Kingdom, 95(2): 289–300. doi: 10.1017/S0025315414000836
    [16]
    Hirai E. 1958. On the species of Cladonema radiatum var. mayeri Perkins. Bulletin of the Marine Biological Station of Asamushi, 9(1): 23–25
    [17]
    Lindner A, Govindarajan A F, Migotto A E. 2011. Cryptic species, life cycles, and the phylogeny of Clytia (Cnidaria: Hydrozoa: Campanulariidae). Zootaxa, 2980(1): 23–36. doi: 10.11646/zootaxa.2980.1.2
    [18]
    Nawrocki A M, Schuchert P, Cartwright P. 2010. Phylogenetics and evolution of Capitata (Cnidaria: Hydrozoa), and the systematics of Corynidae. Zoologica Scripta, 39(3): 290–304. doi: 10.1111/j.1463-6409.2009.00419.x
    [19]
    Östman C. 1979. Two types of nematocysts in Campanulariidae (Cnidaria, Hydrozoa) studied by light and scanning electron microscopy. Zoologica Scripta, 8(1–4): 5–12
    [20]
    Östman C. 1982. Nematocysts and taxonomy in Laomedea, Gonothyraea and Obelia (Hydrozoa, Campanulariidae). Zoologica Scripta, 11(4): 227–241. doi: 10.1111/j.1463-6409.1982.tb00536.x
    [21]
    Östman C. 1987. New techniques and old problems in hydrozoan systematics. In: Bouillon J, Boero F, Cicogna F, et al, eds. Modern Trends in the Systematics, Ecology, and Evolution of Hydroids and Hydromedusae. Oxford: Clarendon Press, 67–82
    [22]
    Östman C. 2000. A guideline to nematocyst nomenclature and classification, and some notes on the systematic value of nematocysts. Scientia Marina, 64(S1): 31–46. doi: 10.3989/scimar.2000.64s131
    [23]
    Purcell J E. 2018. Successes and challenges in jellyfish ecology: examples from Aequorea spp. Marine Ecology Progress Series, 591: 7–27. doi: 10.3354/meps12213
    [24]
    Ralph P M. 1953. A guide to the athecate (gymnoblastic) hydroids and medusae of New Zealand. Tuatara, 5(2): 59–75
    [25]
    Raghu P R, Tampi P, Durve V. 1961. Note on the occurrence of the Anthomedusa cladonema in the Indian region. Journal of the Marine Biological Association of India, 3(1−2): 251–252
    [26]
    Rees W J. 1950. On Cladonema myersi, a new species of hydroid from the Californian Coast. Proceedings of the Zoological Society of London, 119(4): 861–865
    [27]
    Rees J T. 1979. The polyp and immature medusa stages of Cladonema californicum, Hyman, 1947 (Anthomedusae: Cladonemidae) with biological notes and a discussion of the taxonomy of the genus Cladonema. Journal of Natural History, 13(3): 295–302. doi: 10.1080/00222937900770231
    [28]
    Rees J T. 1982. The hydrozoan Cladonema in California: a possible introduction from East Asia. Pacific Science, 36(4): 439–444
    [29]
    Schuchert P. 1996. The marine fauna of New Zealand: athecate hydroids and their medusae (Cnidaria: Hydrozoa). New Zealand Oceanographic Institute Memoir, 106: 1–159
    [30]
    Schuchert P. 2006. The European athecate hydroids and their medusae (Hydrozoa, Cnidaria): Capitata part 1. Revue suisse de Zoologie, 113(2): 325−410.
    [31]
    Schuchert P. 2016. The polyps of Oceania armata identified by DNA barcoding (Cnidaria, Hydrozoa). Zootaxa, 4175(6): 539–555. doi: 10.11646/zootaxa.4175.6.3
    [32]
    Schuchert P. 2021. World Hydrozoa Database. Cladonema Dujardin, 1843. http://marinespecies.org/aphia.php?p=taxdetails&id=117049[2021-09-14]
    [33]
    Schuchert P, Hosia A, Leclère L. 2017. Identification of the polyp stage of three leptomedusa species using DNA barcoding. Revue Suisse de Zoologie, 124(1): 167–182
    [34]
    Takeda N, Deguchi R, Itabashi T. 2018. Reproductive strategies in marine Hydrozoan Jellyfish: sexual medusae and asexual polyps. In: Kobayashi K, Kitano T, Iwao Y, et al, eds. Reproductive and Developmental Strategies. Tokyo: Springer, 157–174
    [35]
    Tamura K, Peterson D, Peterson N, et al. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731–2739. doi: 10.1093/molbev/msr121
    [36]
    Xu Zhenzu. 1993. Revisions of nominal species on the Hydromedusae of China sea areas. Journal of Oceanography in Taiwan Strait, 12(3): 197–204
    [37]
    Zheng Lianming, He Jinru, Lin Yuanshao, et al. 2014. 16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China. Acta Oceanologica Sinica, 33(4): 55–76. doi: 10.1007/s13131-014-0415-8
    [38]
    Zhou Taixuan, Huang Mingxian. 1958. A study on hydromedusae of Chefoo. Acta Zoologica Sinica, 10(2): 173–197
    [39]
    Zhou Konglin, Zheng Lianming, He Jinru, et al. 2013. Detection of a new Clytia species (Cnidaria: Hydrozoa: Campanulariidae) with DNA barcoding and life cycle analyses. Journal of the Marine Biological Association of the United Kingdom, 93(8): 2075–2088. doi: 10.1017/S0025315413000969
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(7)

    Article Metrics

    Article views (354) PDF downloads(35) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return