The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles

Congcong Guo; Guicheng Zhang; Shan Jian; Wei Ma; Jun Sun

doi:10.1007/s13131-023-2182-x

Volume 42 Issue 4

Apr. 2023

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Congcong Guo, Guicheng Zhang, Shan Jian, Wei Ma, Jun Sun. The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles[J]. Acta Oceanologica Sinica, 2023, 42(4): 50-58. doi: 10.1007/s13131-023-2182-x

Citation:

Congcong Guo, Guicheng Zhang, Shan Jian, Wei Ma, Jun Sun. The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles[J]. Acta Oceanologica Sinica, 2023, 42(4): 50-58. doi: 10.1007/s13131-023-2182-x

Citation:

Congcong Guo, Guicheng Zhang, Shan Jian, Wei Ma, Jun Sun. The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles[J]. Acta Oceanologica Sinica, 2023, 42(4): 50-58. doi: 10.1007/s13131-023-2182-x

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The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles

doi: 10.1007/s13131-023-2182-x

Congcong Guo^{1, 2, 3},
Guicheng Zhang³,
Shan Jian³,
Wei Ma⁴,
Jun Sun^{2, 3
,
,}

1.
Institute of Marine Science and Technology, Shandong University, Qingdao 266237, China
2.
Institute for Advanced Marine Research, China University of Geosciences, Guangzhou 511462, China
3.
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China
4.
Huaihe River Basin Eco-environment Monitoring and Scientific Research Center, Huaihe River Basin Eco-environment Supervision and Administration Bureau, Bengbu 233001, China

Funds: The National Key Research and Development Project of China under contract No. 2019YFC1407805; the National Natural Science Foundation of China under contract Nos 41876134, 41676112 and 41276124; the Tianjin 131 Innovation Team Program under contract No. 20180314; the Changjiang Scholar Program of Chinese Ministry of Education under contract No. T2014253.

More Information

Corresponding author: E-mail: phytoplankton@163.com
Received Date: 2020-05-10
Accepted Date: 2020-10-21

Available Online: 2023-03-08

Publish Date: 2023-04-25

Abstract

Abstract

The quantification of transparent exopolymer particles (TEP) by colorimetric method is of large error and low repeatability, one major reason of which is related to the absence of clear definition and evaluation for part steps of the original method. It is obscure that the 80% sulfuric acid solution, acted as the extraction solution in the determination of TEP, is prepared based on a volume ratio or mass ratio. Furthermore, the change of solubility of recently available Gum Xanthan (GX) from the market means that the original protocol is no longer applicable, and the grinding of GX stock solution with a tissue grinder is replaced by shaking with a rotating shaker in the study to prevent the excessive dissolution of GX. We found that different preparation techniques could result in the varied concentrations of 80% H₂SO₄. The duration of shaking during the preparation of standard solution significantly affected the slope of the calibration curve, which caused different correction results of TEP. The impacts of different extraction solution concentrations and shaking time of GX solution on the quantification of TEP were investigated based on the field sampling and laboratory analysis. The extraction capacities of H₂SO₄ with different concentrations for Alcian Blue were distinct, but had limited effect on the final measuring result of TEP. The change of the standard curve slope came along with the variation of shaking time, which markedly altered the detection limit and calibration result, and the extended shaking time was in favor of the determination of low-concentration TEP. It was suggested that the extraction solution concentration, shaking time and filtration volume of standard solution are required to be well controlled and selected to obtain more accurate results for TEP with different concentrations.
- transparent exopolymer particles,
- determination method,
- method improvement,
- sulfuric acid,
- shaking time

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