WU Changwen, CHI Changfeng, HE Guangyuan, LÜ Zhenming, XU Meiying. Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns[J]. Acta Oceanologica Sinica, 2010, (6): 121-124. doi: 10.1007/s13131-010-0083-2
Citation:
WU Changwen, CHI Changfeng, HE Guangyuan, LÜ Zhenming, XU Meiying. Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns[J]. Acta Oceanologica Sinica, 2010, (6): 121-124. doi: 10.1007/s13131-010-0083-2
WU Changwen, CHI Changfeng, HE Guangyuan, LÜ Zhenming, XU Meiying. Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns[J]. Acta Oceanologica Sinica, 2010, (6): 121-124. doi: 10.1007/s13131-010-0083-2
Citation:
WU Changwen, CHI Changfeng, HE Guangyuan, LÜ Zhenming, XU Meiying. Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns[J]. Acta Oceanologica Sinica, 2010, (6): 121-124. doi: 10.1007/s13131-010-0083-2
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China;Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
2.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China
3.
Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China
4.
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni.
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