Volume 39 Issue 12
Jan.  2021
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Zifeng Zhan, Kuidong Xu. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. Acta Oceanologica Sinica, 2020, 39(12): 90-94. doi: 10.1007/s13131-020-1683-0
Citation: Zifeng Zhan, Kuidong Xu. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. Acta Oceanologica Sinica, 2020, 39(12): 90-94. doi: 10.1007/s13131-020-1683-0

Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization

doi: 10.1007/s13131-020-1683-0
Funds:  The National Natural Science Foundation of China under contract Nos 41876171 and 41406171.
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  • Corresponding author: E-mail: kxu@qdio.ac.cn
  • Received Date: 2020-02-14
  • Accepted Date: 2020-07-14
  • Available Online: 2021-04-21
  • Publish Date: 2020-12-25
  • The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs.
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