Simultaneous nitrification and denitrification conducted by <i>Halomonas venusta</i> MA-ZP17-13 under low temperature conditions

Guizhen Li; Qiliang Lai; Guangshan Wei; Peisheng Yan; Zongze Shao

doi:10.1007/s13131-021-1897-9

Volume 40 Issue 9

Sep. 2021

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Article Navigation > Acta Oceanologica Sinica > 2021 > 40(9): 94-104

Guizhen Li, Qiliang Lai, Guangshan Wei, Peisheng Yan, Zongze Shao. Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions[J]. Acta Oceanologica Sinica, 2021, 40(9): 94-104. doi: 10.1007/s13131-021-1897-9

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Guizhen Li, Qiliang Lai, Guangshan Wei, Peisheng Yan, Zongze Shao. Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions[J]. Acta Oceanologica Sinica, 2021, 40(9): 94-104. doi: 10.1007/s13131-021-1897-9

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Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions

doi: 10.1007/s13131-021-1897-9

Guizhen Li^{1, 2, 3, 4},
Qiliang Lai^{2, 3, 4},
Guangshan Wei^{2, 3, 4, 5},
Peisheng Yan¹,
Zongze Shao^{1, 2, 3, 4, 5
,
,}

1.
School of Environment, Harbin Institute of Technology, Harbin 150090, China
2.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
3.
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, China
4.
Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, China
5.
Sourthern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China

Funds: The COMRA Program under contract No. DY135-B2-01; the Xiamen Ocean Economic Innovation and Development Demonstration Project under contract No. 16PZP001SF16; the National Infrastructure of Natural Resources for Science and Technology Program of China under contract No. NIMR-2017-9.

More Information

Corresponding author: E-mail: shaozz@163.com
Received Date: 2020-10-23
Accepted Date: 2021-02-19
Publish Date: 2021-09-30

Abstract

Abstract

Nitrification is a key step in the global nitrogen cycle. Compared with autotrophic nitrification, heterotrophic nitrification remains poorly understood. In this study, Halomonas venusta MA-ZP17-13, isolated from seawater in shrimp aquaculture (Penaeus vannamei), could simultaneously undertake nitrification and denitrification. With the initial ammonium concentration at 100 mg/L, the maximum ammonium-nitrogen removal rate reached 98.7% under the optimal conditions including C/N concentration ratio at 5.95, pH at 8.93, and NaCl at 2.33%. The corresponding average removal rate was 1.37 mg/(L·h) (according to nitrogen) in 3 d at 11.2°C. By whole genome sequencing and analysis, nitrification- and denitrification-related genes were identified, including ammonia monooxygenase, nitrate reductase, nitrite reductase, nitric oxide dioxygenase and nitric oxide synthase; while no gene encoding hydroxylamine oxidase was identified, it implied the existence of a novel nitrification pathway from hydroxylamine to nitrate. These results indicate heterotrophic bacterium H. venusta MA-ZP17-13 can undertake simultaneous nitrification and denitrification at low temperature and has potential for ${\rm {NH}}_4^+ $-N/NH₃-N removal in marine aquaculture systems.
- Halomonas,
- heterotrophic nitrification,
- denitrification,
- ammonia oxidization,
- ammonia removal

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References(56)

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